Antimicrobial Resistance & CFU Testing

Independent microbiological testing evaluating antimicrobial resistance of Derma Compress over a 20-day simulated use cycle.

Testing Overview

Testing Laboratory:
Pharmichem Testing Services, Inc.
17501 W. Duvan Drive
Tinley Park, Illinois 60477

Protocol Number:
M-018-03

Study Title:
Determination of Antimicrobial Resistance of Derma Compress Against Staphylococcus aureus and Pseudomonas aeruginosa Over a 20 Day Exposure Period CFU Testing #3.

Testing Date:
June / July 2018 CFU Testing #3.

Product Tested:
Derma Compress – 2x10 Vented

Purpose of the Study

This study was conducted to evaluate whether repeated exposure to common wound pathogens would result in cumulative bacterial retention or reduced sanitization effectiveness over time.

The organisms selected for testing were:

  • Staphylococcus aureus

  • Pseudomonas aeruginosa

These organisms are commonly associated with skin infections and biofilm formation.

Testing Method

Exposure Cycle

  • 24-hour exposure periods

  • Organism inoculation

  • Surface contact simulation

Cleaning Process

  • Washed per manufacturer instructions

  • Antibacterial soap

  • Warm water rinse

Repeated Testing

  • CFU enumeration

  • 20 successive exposure days

  • Baseline comparison

20-Day CFU Results

Baseline Measurements:

  • Staphylococcus aureus 38 CFU

  • Pseudomonas aeruginosa: 48 CFU

Observed Results Over 20 Days:

  • CFU counts remained under 100

  • No persistent colonization was observed

  • No evidence of long-term bacterial retention

A temporary spike occurred during testing when handled without gloves; counts returned to baseline following proper cleaning.

Study Conclusion

The Vented Derma Compress device remained resistant to Staphylococcus aureus and Pseudomonas aeruginosa over a 20-day use and cleaning cycle.

Testing indicates repeated exposure and cleaning did not result in cumulative microbial retention under laboratory conditions.

Technical Data

View Full CFU Data by Exposure Day

  • Baseline
    Staphylococcus aureus: 38 CFU
    Pseudomonas aeruginosa: 48 CFU

    Day 1
    Staphylococcus aureus: 9 CFU
    Pseudomonas aeruginosa: 3 CFU

    Day 2
    Staphylococcus aureus: 7 CFU
    Pseudomonas aeruginosa: 37 CFU

    Day 3
    Staphylococcus aureus: 23,000 CFU*
    Pseudomonas aeruginosa: 20,000 CFU*

    Day 4
    Staphylococcus aureus: 2 CFU
    Pseudomonas aeruginosa: 11 CFU

    Day 5
    Staphylococcus aureus: 121 CFU
    Pseudomonas aeruginosa: 460 CFU

    *Day 3 spike associated with handling variation during testing.

  • Day 6
    Staphylococcus aureus: 6 CFU
    Pseudomonas aeruginosa: 200 CFU

    Day 7
    Staphylococcus aureus: 373 CFU
    Pseudomonas aeruginosa: 328 CFU

    Day 8
    Staphylococcus aureus: 69 CFU
    Pseudomonas aeruginosa: 54 CFU

    Day 9
    Staphylococcus aureus: 33 CFU
    Pseudomonas aeruginosa: 15 CFU

    Day 10
    Staphylococcus aureus: 45 CFU
    Pseudomonas aeruginosa: 43 CFU

    Day 11
    Staphylococcus aureus: 7 CFU
    Pseudomonas aeruginosa: 30 CFU

    Day 12
    Staphylococcus aureus: 3,136 CFU
    Pseudomonas aeruginosa: 1,066 CFU

  • Day 13
    Staphylococcus aureus: 17 CFU
    Pseudomonas aeruginosa: 19 CFU

    Day 14
    Staphylococcus aureus: 44 CFU
    Pseudomonas aeruginosa: 341 CFU

    Day 15
    Staphylococcus aureus: 18 CFU
    Pseudomonas aeruginosa: 151 CFU

    Day 16
    Staphylococcus aureus: 23 CFU
    Pseudomonas aeruginosa: 40 CFU

    Day 17
    Staphylococcus aureus: 142 CFU
    Pseudomonas aeruginosa: 18 CFU

    Day 18
    Staphylococcus aureus: 26 CFU
    Pseudomonas aeruginosa: 29 CFU

    Day 19
    Staphylococcus aureus: 16 CFU
    Pseudomonas aeruginosa: 5 CFU

    Day 20
    Staphylococcus aureus: 21 CFU
    Pseudomonas aeruginosa: 43 CFU

Download Full Laboratory Report

Disclaimer

Results reflect laboratory testing conditions and the specific product configuration evaluated.

CFU levels may vary based on real-world handling, environmental conditions, and hygiene practices.

This information is provided for educational and informational purposes and does not constitute a medical claim.